Blood cancer molecular assays

Why choose our products?

Comprehensive line of Real-Time PCR kits: deliver reproducible results, assay after assay.
Standardized reagents and protocols (mostly developed in colllaboration with international networks of experts like Europe Against Cancer (EAC), European LeukemiaNet (ELN), rigorously designed for high performance.
Compliance with international recommandations.
Compatible with most RQ-PCR platforms: fit seamlessly into your workflow.
Knowledgeable, expert sales and technical resources: provide the support you mayneed.
Ready-to-use, pre-diluted solutions: easy to adopt assays and reduce risk of contamination.
Stable, validated plasmid standards: generate accurate standard curves, allowing precise and reproducible quantification of target genes copy numbers in samples.
Fast, timely access to essential new tests of the highest potential value, such as our exclusive JAK2 assays and our new generation of BCR ABL Mbcr IS MMR products.
Products manufactured under ISO 13485 : 2003 certification.
Complete validated solution with analytical performances assessed: to help laboratories in ISO 15189 accreditation process.

Real-Time quantitative RQ-PCR BCR-ABL_NCN results converted to the International Scale Reporting on the MMR status EAC technology	Primers and probe mixes for BCR-ABL & ABL Single Plasmid dilutions including BCR-ABL & ABL IS MMR Calibrator (RNA) High Positive Control (RNA) RT & RQ enzymes included in DX kit

Real-Time quantitative PCR Based on EAC standardized technology Calibrated and accurate quantification of specific fusion gene transcripts, normalized with control gene expression	Primers and probe mixes specific for the fusion gene of interest and for the control gene Plasmid standards specific for the fusion gene of interest and for the control gene (calibrated concentrations)

Allele specific RQ-PCR technology Calibrated and accurate quantification of a specific mutation, relative to wild type or to control gene expression Limit Of Detection (LOD) = 0.06% for JAK2 V617F quantification and 0.009 or 0.008 NCN for NPM1 mut A or B&D quantification respectively	Primers and probe mixes specific for the selected mutant and for the wild type alleles or the control gene Plasmid standards specific for the selected mutant and for the wild type alleles or the control gene (calibrated concentrations)

Allele specific RQ-PCR technology on genomic DNA Sensitive and highly specific detection of the selected mutation Qualitative ΔΔCt analysis Cut-off for positivity at 1% for JAK2 V617F	Primers and probe mixes specific for either the mutant allele or the wild type allele Positive control (100% mutant) and Negative control (0% mutant) Cut-off sample (COS): 1% JAK2 V617F

For JAK2 V617F and for MPL W515L/K:

For NPM1 mutations:

Real-Time PCR Clamp technology on genomic DNA
NPM1 mutational status detection (mutated or wild type) and NPM1 mutation type (A, B or D) identification

For JAK2 V617F and for MPL W515L/K:

Single tubes with mixed primers and probes specific for the mutation or the wild type detection
Positive control (100% mutant) and Negative control (0% mutant)
Cut-off samples (COS): 2% for JAK2 V617F or 1.5% for MPL W515L/K
Reference scale in JAK2 MSPP-03-CE kit (dilutions of genomic DNA for semi- quantification)
RQ enzyme included in EZ kit

For NPM1 mutations:

5 primers and probe mixes specific for Total NPM1, Mutated NPM1, NPM1 Mut A, Mut B and Mut D separately
NPM1 Mut A/B/D Positive Control and NPM1 wild type Control