SIL-TAL

• About this biomarker
• Introduction to our products
• Why choose our products
• Main product for SIL-TAL
• Associated products
• Related publications

The microdeletion on 1p32 is the most frequent chromosome aberration found in childhood T-ALL.

The microdeletion involves the TAL1 gene (T-cell acute leukemia 1 gene) and the SIL gene (SCL interrupting locus), which is located approximately 90 kb upstream.

SIL-TAL1 transcripts are exclusively found in T-ALL, in which they are present in 5-25% of the patients.

The SIL-TAL1 fusion gene transcripts seem to be more frequent in children compared to adults.

SIL-TAL FusionQuant® technology uses Real-Time Quantitative RQ-PCR to quantify the expression level of specific E2A-PBX1 fusion gene transcripts relative to ABL control gene.

Quantification of SIL-TAL fusion gene transcripts has been standardized in the EAC (Europe Against Cancer) program  [Gabert J et al. Leukemia. 2003, Beillard E et al. Leukemia. 2003], and IPSOGEN FusionQuant® kits use this validated technology to calibrate and normalize RQ-PCR results.

Why choose SIL-TAL FusionQuant® Standards?

• EAC Standardized RQ-PCR procedures

• Compatibility with most RQ-PCR platforms

• Calibrated and sensitive quantification of fusion gene transcript, normalized with ABL (BCR or GUS) control gene (results in NCN)

• Ready-to-Use plasmid  standards

Standardization and quality control studies of 'real-time' quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia - a Europe Against Cancer program.
Leukemia. 2003
Authors: Gabert J et al.

Evaluation of candidate control genes for diagnosis and residual disease detection in leukemic patients using 'real-time' quantitative reverse-transcriptase polymerase chain reaction (RQ-PCR) - a Europe against cancer program.
Leukemia. 2003
Authors: Beillard E et al.